Journal: Aging (Albany NY)

Article Title: Ambient particulate matter attenuates Sirtuin1 and augments SREBP1-PIR axis to induce human pulmonary fibroblast inflammation: molecular mechanism of microenvironment associated with COPD

doi: 10.18632/aging.102077

Figure Lengend Snippet: Signatures regulated by PM in HPF models. ( A ) The mRNA level of SREBP1 after PM treatment. ( B ) The prediction of SREBP1 -network derived from the common signature, by comparison of IPA database with microarray data from HPF cells with a 1.5-fold-change cut-off. The intensity of the node color indicates the degree of activating (orange) or inhibiting (blue) regulation following PM interaction. ( C ) Several mRNA levels of SREBP1 downstream factors, including PIR, LIF, IL-6, IL-24, and CXCL8, after PM treatment in HPF.

Article Snippet: Western blot analysis was performed with primary anti-PIR antibody (GeneTex, Irvine CA, USA), anti-NLRP3 antibody (GeneTex, Irvine, CA, USA), anti-SREBP1 antibody (GeneTex, Irvine CA, USA), anti-IL-6 antibody (Abcam, Cambridge MA, USA), anti-IL-1β antibody (GeneTex, Irvine, CA, USA), anti-SIRT1 antibody (Proteintech®, Rosemont, USA), and anti-β-actin antibody (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Derivative Assay, Comparison, Microarray

Journal: Aging (Albany NY)

Article Title: Ambient particulate matter attenuates Sirtuin1 and augments SREBP1-PIR axis to induce human pulmonary fibroblast inflammation: molecular mechanism of microenvironment associated with COPD

doi: 10.18632/aging.102077

Figure Lengend Snippet: PM exposure is positively correlated with activation of the SREBP1-PIR signaling pathway through SIRT1 downregulation. ( A ) Western blot analysis of PIR after PM treatment in HPF. ( B ) PIR activity from cell culture supernatant in PM-induced models. ( C ) Levels of SREBP1, SIRT1, and PIR were measured from cell culture supernatant of PM-induced models. ( D ) mRNA levels of SREBP1 in PF429242-PM co-treated HPF models. ( E ) mRNA levels of PIR in PF429242-PM co-treated HPF models. ( F ) PIR activity from cell culture supernatant in PF429242-PM co-treated HPF models. ( G ) Western blot analysis of PIR and SIRT1 in TPh A-PM co-treated HPF models. ( H ) PIR activity from cell culture supernatant in TPh A-PM co-treated HPF models. ( I ) mRNA level of PIR in Ex527-PM co-treated HPF models. ( J ) Western blot analysis of SIRT1 and PIR in Ex527-PM co-treated HPF models. ( K ) Level of PIR was measured from cell culture supernatant in Ex527-PM co-treated HPF models.

Article Snippet: Western blot analysis was performed with primary anti-PIR antibody (GeneTex, Irvine CA, USA), anti-NLRP3 antibody (GeneTex, Irvine, CA, USA), anti-SREBP1 antibody (GeneTex, Irvine CA, USA), anti-IL-6 antibody (Abcam, Cambridge MA, USA), anti-IL-1β antibody (GeneTex, Irvine, CA, USA), anti-SIRT1 antibody (Proteintech®, Rosemont, USA), and anti-β-actin antibody (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Activation Assay, Western Blot, Activity Assay, Cell Culture

Journal: Aging (Albany NY)

Article Title: Ambient particulate matter attenuates Sirtuin1 and augments SREBP1-PIR axis to induce human pulmonary fibroblast inflammation: molecular mechanism of microenvironment associated with COPD

doi: 10.18632/aging.102077

Figure Lengend Snippet: NLRP3 inflammasome was upregulated by PM exposure. ( A ) mRNA level of NLRP3 after PM in HPF. ( B ) Western blot analysis of NLRP3 and IL-1β after PM in HPF. ( C ) mRNA level of NLRP3 in Ex527-PM co-treated HPF models. ( D ) mRNA levels of NLRP3 in PF429242-PM co-treated HPF models. ( E ) Western blot analysis of PIR, SREBP1, and NLRP3 in TPh A-PM co-treated HPF models.

Article Snippet: Western blot analysis was performed with primary anti-PIR antibody (GeneTex, Irvine CA, USA), anti-NLRP3 antibody (GeneTex, Irvine, CA, USA), anti-SREBP1 antibody (GeneTex, Irvine CA, USA), anti-IL-6 antibody (Abcam, Cambridge MA, USA), anti-IL-1β antibody (GeneTex, Irvine, CA, USA), anti-SIRT1 antibody (Proteintech®, Rosemont, USA), and anti-β-actin antibody (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Western Blot

Journal: Aging (Albany NY)

Article Title: Ambient particulate matter attenuates Sirtuin1 and augments SREBP1-PIR axis to induce human pulmonary fibroblast inflammation: molecular mechanism of microenvironment associated with COPD

doi: 10.18632/aging.102077

Figure Lengend Snippet: A model illustrates that PM activates the SREBP1-PIR/inflammasomes signaling axis through SIRT1 -mediated modulation. ( A ) A schematic model to describe the positive feedback loop and detailed mechanisms of the SIRT1-SREBP1-PIR axis and crosstalk with NF-κB signaling. ( B ) SIRT1 modulates the SREBP1-PIR/NLRP3 inflammasome axis in response to PM. SIRT1 functions as a protector from PM exposure. The SIRT1-SREBP1-PIR/NLRP3 inflammasome axis may present an attractive therapeutic target for PM-related adverse health events.

Article Snippet: Western blot analysis was performed with primary anti-PIR antibody (GeneTex, Irvine CA, USA), anti-NLRP3 antibody (GeneTex, Irvine, CA, USA), anti-SREBP1 antibody (GeneTex, Irvine CA, USA), anti-IL-6 antibody (Abcam, Cambridge MA, USA), anti-IL-1β antibody (GeneTex, Irvine, CA, USA), anti-SIRT1 antibody (Proteintech®, Rosemont, USA), and anti-β-actin antibody (Sigma-Aldrich, St. Louis, MO, USA).

Techniques: